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Advanced methods for cardiac cells contour detection
Spíchalová, Barbora ; Čmiel, Vratislav (referee) ; Odstrčilík, Jan (advisor)
This thesis focuses on advanced methods of detecting contours of the cardiac cells and measuring their contraction. The theoretical section describes the types of confocal microscopes, which are used for capturing biological samples. The following chapter is devoted to the methods of cardiac cells segmentation, where we are introduced to the generally applied approaches. The most widely spread methods of segmentation are active contours and mathematical morphology, which are the crucial topics of this thesis. Thanks to the those methods we are able in the visual data to accurately detect required elements and measure their surface chnage in time. Acquired theoretical knowledge leads us to the practical realization of the methods in MATLAB.
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Utilization of confocal microscopy to study of degradation of organic dye in cell biology
Trnová, Kateřina ; Provazník, Ivo (referee) ; Čmiel, Vratislav (advisor)
This thesis focuses on the study of the life of a fluorescent dye. It also examines changes in its emission spectrum determined with the aid of development of fluorescence intensity over the long term sensing in several kinds if of cella. The general part of the thesis with the issues of confocal microscopy, fluorescent radiation and maks. Furthermorer, an analysis of methods for measuring fluorescence lifetime is done. In the thesis there is elaborated a description of the program, whitch was designed for the analysis of the collected output of the used confocal microscope. Sebsequently, the results are evaluated.
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Software FLIM system in confocal microscopy
Petrula, Jakub ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This bachelor thesis deals with the FLIM system utilization in confocal microscopy. It describes basic concepts which are closely related to the topic, such as principles of fluorescence, fluorescent dyes and principles of both fluorescence and confocal microscopy. Practical part involves work with the confocal microscope and set of intensity images acquisition of both the animal and plant cells. Final product of pratical part is fluorescence lifetime image. The thesis also describes an algorithm for generating a pseudocolor frames and created user interface.
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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Design of microscope LED illumination system
Gricová, Monika ; Dostál, Zbyněk (referee) ; Antoš, Martin (advisor)
The objective of this bachelor thesis was to design illumination assemblage in confocal microscope Confocal 2002. First part of this paper describes types and components of confocal microscopes. For correct function of the microscope, high-power illumination by a white light emmiter is required. Based on the analysis of the background research, suitable LED diode was selected. In experimental part of this work, eight optical systems are proposed, that has been assembled and evaluated. Based on the evaluation of obtained paramteres, the optimal assemblage was selected and designed for production.
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Design of microscope LED illumination system
Gricová, Monika ; Dostál, Zbyněk (referee) ; Antoš, Martin (advisor)
The objective of this bachelor thesis was to design illumination assemblage in confocal microscope Confocal 2002. First part of this paper describes types and components of confocal microscopes. For correct function of the microscope, high-power illumination by a white light emmiter is required. Based on the analysis of the background research, suitable LED diode was selected. In experimental part of this work, eight optical systems are proposed, that has been assembled and evaluated. Based on the evaluation of obtained paramteres, the optimal assemblage was selected and designed for production.
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Software FLIM system in confocal microscopy
Petrula, Jakub ; Odstrčilík, Jan (referee) ; Čmiel, Vratislav (advisor)
This bachelor thesis deals with the FLIM system utilization in confocal microscopy. It describes basic concepts which are closely related to the topic, such as principles of fluorescence, fluorescent dyes and principles of both fluorescence and confocal microscopy. Practical part involves work with the confocal microscope and set of intensity images acquisition of both the animal and plant cells. Final product of pratical part is fluorescence lifetime image. The thesis also describes an algorithm for generating a pseudocolor frames and created user interface.
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